Volume 20, Issue 6 (9-2017)                   J Arak Uni Med Sci 2017, 20(6): 74-82 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Matini M, Bakhtiarnejad S, Dastan D, Maghsood A H, Fallah M. In-Vitro Efficacy of Plantago lanceolata L. Extracts on Trichomonas Vaginalis. J Arak Uni Med Sci. 2017; 20 (6) :74-82
URL: http://amuj.arakmu.ac.ir/article-1-5089-en.html
Assistant Professor, PhD in Parasitology, Department of Medical Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran , matini@umsha.ac.ir
Abstract:   (1337 Views)
Abstract
Background: Trichomoniasis is one of the most common non viral sexually transmitted diseases worldwide. The aim of this study was to investigate the effect of Plantago lanceolata extracts on Trichomonas vaginalis.
Materials and Methods: In this study, after collection and drying of P. lanceolata, n-hexanic, ethyl acetate, methanol and hydroalcoholic extracts, they were prepared by maceration. Five clinical T. vaginalis isoleates subjected to extract suscebtibility testing, in comparison of metronidazole. Minimum inhibitory concentration (MIC) and minimum lethal concentration (MLC) tests were carried out in duplicate and repeated two times for each T. vaginalis isolate.
Results: The results showed that the extracts of P. lanceolata had potent antitrichomonal activity. The most antitrichomonal activity was related to ethyl acetate extract with the least MIC of 500 µg/ml and mean of 1525 µg/ml, after 48 hrs incubation. And also, the lowest antitrichomonal activity was related to hydroalcoholic and methanolic extract with the least and mean MIC of 2000 µg/ml. The results of MLC and MIC tests were identical and this finding confirmed the trichomonacidal activity of the extracts. The drug suscebtibility testing showed that the T. vaginalis isoleates were susceptibale to metronidazole ranging from 3.1 to 6.2 µg/ml with a mean and standard deviation of 4.2 ± 1.5 µg/ml.
Conclusion: This study showed that the extracts of P. lanceolata hav e a considerable activity on T. vaginalis parasite. Hence, further studies are needed to clear more details of antimicrobial properties of  P. lanceolata compounds.
Full-Text [PDF 773 kb]   (527 Downloads)    
Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2017/05/7 | Accepted: 2017/08/2 | Published: 2017/08/21

Add your comments about this article : Your username or Email:
CAPTCHA code

Send email to the article author


© 2018 All Rights Reserved | Journal of Arak University of Medical Sciences

Designed & Developed by : Yektaweb