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:: Volume 20, Issue 1 (4-2017) ::
AMUJ 2017, 20(1): 1-13 Back to browse issues page
Morphine and Naloxone Effects on Seizure in Brain Slice of Morphine-Dependent Infant Mice
Yousef Panahi , Ehsan Sabori , Ali Rasouli , Goodarz Sadeghi Hashjin , Shiva Roshan Milani , Leila Derafshpour
Professor, Department of Physiology, Urmia University of Medical Sciences, Urmia, Iran , e.saboory@yahoo.com
Abstract:   (1098 Views)

Abstract

Background: The aim of this study was to evaluate the effects of different concentrations of morphine and naloxone on epileptic activity in live brain slices taken from morphine-dependent and control infant mice.

Materials and Methods: Forty neonatal mice were randomly selected. To establish dependency, 2, 4, 8, 16 and 32 mg / kg morphine was injected subcutaneously once daily (0.1 cc) for 5 consecutive days from day 14-18 after birth. On postnatal days 19-20, brain slices were prepared and cerebrospinal fluid was perfused with low magnesium to induce experimental- epiletform activity. The effects of 10, 100 and 1000 µM concentrations of morphine and 10 µM naloxone were investigated on epileptic activity. Chenges in the number as well as onset and amplitude of activities were considered as an indicator to determine the quantity of their effect.  

Results: The results showed that morphine 100 µM increased the activity while 10 and 1000 µM concentrations of morphine and 10 µM naloxone attenuated epileptic activity in both groups. Naloxone reduced pro-seizure effect of morphine, but anti-seizure effect of morphine couldn't restored by naloxone.

Conclusion: Morphine has a two-phase concentration-dependent effect on epileptic activity in the infant mice; so that low and high concentrations of morphine inhibit epileptic activity, but its moderate concentration potentiates the epileptic activity. Naloxone has an anti-seizure effect.

Keywords: Brain slices, Mice, Morphine, Naloxone, Seizure
Full-Text [PDF 640 kb]   (493 Downloads)    
Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2017/02/8 | Accepted: 2017/02/15 | Published: 2017/03/14
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Panahi Y, Sabori E, Rasouli A, Sadeghi Hashjin G, Roshan Milani S, Derafshpour L. Morphine and Naloxone Effects on Seizure in Brain Slice of Morphine-Dependent Infant Mice. AMUJ. 2017; 20 (1) :1-13
URL: http://amuj.arakmu.ac.ir/article-1-4936-en.html


Volume 20, Issue 1 (4-2017) Back to browse issues page
مجله دانشگاه علوم پزشکی اراک Arak Medical University Journal
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