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:: Volume 19, Number 12 (3-2017) ::
amuj 2017, 19(12): 70-77 Back to browse issues page
Evaluation of Anti-Oxidant Activity of Lavandula angustifolia using DPPH Method
Masoud Soheili, Mohammad Ali Khandan, Mahmoud Salami
Professor, PhD of Physiology, Physiology Research Center, Kashan University of Medical Sciences, Kashan, Iran , salami-m@kaums.ac.ir
Abstract:   (1100 Views)

Abstract

Background: Stress oxidative factors are known to causes diseases resulting from metabolic disorders. Therefore, preventing, or at least decreasing the amount of these factors may have a positive impact on prevention or improvement of the metabolic problems. Recently, the herbal medicines are more considered due to more effectiveness. We designed the present study to evaluate anti-oxidant effect of aqueous extract of lavender (Lavandula angustifolia).

Materials and Methods: For extract preparation, the dry aerial part of lavender mixed with boiling water for 4 hours and then the container was filtered and condensed in a bain marie. Finally, the extract was powdered by freeze dryer. The anti-oxidant activities of the herbal medicine samples in 5, 10, 20 and 40 ppm concentrations were determined via DPPH method. This method is based on free radical scavenging of 2, 2- dipheny L-1-picrylhydrazyl(DPPH) reflected in the color and absorbance changes in spectrophotometery method in 520 nm. Finally the IC50 was calculated and compared with that of for vitamin C as a standard.

Results: All doses of the aqueous extract of the lavender showed dose- dependent potent anti-oxidant activity, So that, their differences were significant compared to control sample. The IC50 of the herbal medicine was 24.66 ppm that was less than the vitamin C of 2.3 ppm.

Conclusion: As a potent anti-oxidant, the lavender aqueous extract can be effective in treatment of metabolic diseases.

Keywords: Anti-oxidant, Aqueous extract, Lavandula angustifolia, Metabolic disorders
Full-Text [PDF 602 kb]   (1385 Downloads)    
Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2016/10/27 | Accepted: 2016/12/7 | Published: 2017/02/14
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Soheili M, Khandan M A, Salami M. Evaluation of Anti-Oxidant Activity of Lavandula angustifolia using DPPH Method. amuj. 2017; 19 (12) :70-77
URL: http://amuj.arakmu.ac.ir/article-1-4742-en.html
Volume 19, Number 12 (3-2017) Back to browse issues page
مجله دانشگاه علوم پزشکی اراک Arak Medical University Journal
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