Volume 12, Issue 4 (2-2010)                   J Arak Uni Med Sci 2010, 12(4): 105-112 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mehrabi nasab E, Khazaei M, Khazaei S. The effect of pentylenetetrazol kindling induced epilepsy on hypogonad hormones and sperm parameters of rats. J Arak Uni Med Sci. 2010; 12 (4) :105-112
URL: http://amuj.arakmu.ac.ir/article-1-396-en.html
medicine , entezar148@yahoo.com
Abstract:   (15162 Views)
Background: Clinical research suggest a relationship between epilepsy and hypogonadism. The aim of this study is to identify the impact of epilepsy induced by kindling with pentylenetetrazol (PTZ) on hormones and some reproductive parameters in male rats. Materials and Methods: Adult male rats (NMRI strain) were divided into control and kindling groups. Kindling was induced by 40 mg/kg PTZ with a 48 hour interval through intraperitoneal injection. Each animal received 13 doses. At the end of the thirteenth dose, animals were weighed and uthenaized with ether and blood samples, collected from their hearts, were used for testosterone, prolactin and FSH, LH assay. In order to examine motility and morphology of sperms, tissue samples from epidydimis were isolated and minced in DMEM/F12 culture medium and were incubated for 15 minutes. Then sperm morphology and motility were studied. Testis were also isolated and weighed. Results: Kindling with PTZ decreased serum levels of testosterone and LH, but it increased rolactin. However, there was no difference in serum level of FSH between the two groups. Sperm motility in kindling group decreased significantly. There were no significant differences in testis weight, but the amount of animal weight losses in the kindling group was more than that of the control group. Conclusion: By changing the serum level of sexual hormones and decreasing sperm motility, PTZ kindling induced epilepsy exerts diminishing negative effects on reproduction.
Full-Text [PDF 379 kb]   (1642 Downloads)    
Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2009/07/14

Add your comments about this article : Your username or Email:
Write the security code in the box

Send email to the article author

© 2018 All Rights Reserved | Journal of Arak University of Medical Sciences

Designed & Developed by : Yektaweb