[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 18, Number 12 (3-2016) ::
amuj 2016, 18(12): 82-92 Back to browse issues page
Application of the Nano Polyamidoamine –G5 (NPAMAM-G5)Dendrimer in Removal of Escherichia Coli, Klebsiella Oxytoca,Pseudomonas Aeruginosa, Proteus Mirabilis and Staphylococcus Aureus from Aqueous Medium
Shahram Nazari, Sajjad Ashkani, Hadi Yousefzadeh, Fazel Agaei, Gharib Majidi, Aziz Kamran, Payman Azghani, Ayyob Rastegar, Zohreh Nazari, Soudabeh Alizadeh Matboo
BS, Student Research Committee, Faculty of Health, Ardabil University of Medical Sciences, Ardabil, Iran , Soudabehalizadeh@ymail.com
Abstract:   (2234 Views)

Background: Dendrimers are macromolecules with regular three-dimensional structure that have many branches. The aim of this study was to investigate the fficiency of Nano Polyamidoamine–G5 (NPAMAM-G5) dendrimers in removal of Escherichia coli, Klebsiella oxytoca, Pseudomonas aeruginosa, Proteus mirabilis and Staphylococcus aureus from aqueous solution.

Materials and Methods: In this experimental study, initially, dilution of 103 CFU/ml was prepared from each strain of bacteria. Then, different concentrations of dendrimers (0.025, 0.25, 2.5 and 25 µg/ml) in the ambient temperature (23-25°C) was added to water. In order to determine the efficiency of dendrimers in removal of bacteria, samples were taken at different times (0, 10, 20, 30, 50 and 60 min) and were cultured on nutrient agar medium. Samples were incubated for 24 hours at 37 ° C and then the number of colonies was counted.

Results: There is a direct relation between antibacterial properties of dendrimers in aqueous solution and increasing the dendrimer concentration and contact time. At a concentration of 25 μg/ml and 60 minutes, all kinds of bacteria except Staphylococcus aureus, and at 30 minutes, E. coli and Klebsiella bacteria, were removed by 100% The concentration of 2.5 μg/ml at 60 minutes of  bacteria, Escherichia coli, Klebsiella and Proteus mirabilis, 100% Were removed. All concentrations of dendrimer at different times were reduced bacteria.

Conclusion: Results of this study indicated that NPAMAM–G5 dendrimer is able to remove Escherichia coli, Klebsiella oxytoca, Pseudomonas aeruginosa, Proteus mirabilis and Staphylococcus aureus from aqueous solution.

Keywords: Bacteria, Antibacterial properties, Aqueous solution, Nano Polyamidoamine–G5 Dendrimer
Full-Text [PDF 809 kb]   (474 Downloads)    
Type of Study: Original Atricle | Subject: Health
Received: 2015/08/30 | Accepted: 2015/11/9 | Published: 2016/02/22
Send email to the article author

Add your comments about this article
Your username or email:

Write the security code in the box >



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Nazari S, Ashkani S, Yousefzadeh H, Agaei F, Majidi G, Kamran A, et al . Application of the Nano Polyamidoamine –G5 (NPAMAM-G5)Dendrimer in Removal of Escherichia Coli, Klebsiella Oxytoca,Pseudomonas Aeruginosa, Proteus Mirabilis and Staphylococcus Aureus from Aqueous Medium . amuj. 2016; 18 (12) :82-92
URL: http://amuj.arakmu.ac.ir/article-1-3936-en.html
Volume 18, Number 12 (3-2016) Back to browse issues page
مجله دانشگاه علوم پزشکی اراک Arak Medical University Journal
Persian site map - English site map - Created in 0.061 seconds with 809 queries by yektaweb 3503