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:: Volume 18, Issue 7 (10-2015) ::
AMUJ 2015, 18(7): 66-74 Back to browse issues page
Evaluation of the Aassociation Between Programmed Cell Death 1 Gene Single Nucleotide Polymorphism +7146A/G and Susceptibility to Chronic Hepatitis B
Seyed Reza Mohebbi , Hamed Naghoosi , Pedram Azimzadeh , Shaghayegh Derakhshani , Afsaneh Sharifian , Mohammad Reza Zali
Assistant professor Basic Sciences and Epidemiology of Gastroenterology Diseases Research Center, Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Siences, Tehran, Iran. , srmohebbi@gmail.com
Abstract:   (2776 Views)

  Background: In spite of designing and applying an effective vaccine against Hepatitis B virus (HBV), chronic infection with this virus is still one of the most important health problems worldwide. Host genetic background including single nucleotide polymorphisms play a significant role in chronicity or clearance of the infection. The final product of programmed cell death 1 gene (PDCD1) is expressed frequently on T-cells and in chronic viral infections, prevent the virus-specific T-cell response against the virus. In this study, the association of a single nucleotide polymorphism (+7146A/G) in intron 4 of PD1 gene with chronic hepatitis B infection in Iranian population has been assessed.

  Materials and Methods: 212 chronic HBV patients and 208 healthy controls were analyzed in this case-control study. Genomic DNA of the studied individuals was extracted and after performing polymerase chain reaction (PCR), polymorphism of +7146 was determined via RFLP method.

  Results: Frequencies of GG, GA and AA genotypes on position 7146 of the intron 4 of PD1 gene were 77.4%, 20.7% and 1.9% in patient group and 80.8%, 15.4% and 3.8% in control group, respectively. After statistical analysis, No significant difference was observed between patient and control groups (p=0.198).

  Conclusion: Genotype frequencies in the studied population are in accordance with the results of previous studies. Results of the present study suggest that there is not any association between A/G single nucleotide polymorphism in intron 4 of PD1 gene and susceptibility to chronic hepatitis B in Iranian population.

Keywords: Chronic infection, Hepatitis B, Human PDCD1 protein, Polymorphism, Single Nucleotide
Full-Text [PDF 654 kb]   (532 Downloads)    
Type of Study: Original Atricle | Subject: Infection
Received: 2015/03/26 | Accepted: 2015/05/20 | Published: 2015/09/22
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Mohebbi S R, Naghoosi H, Azimzadeh P, Derakhshani S, Sharifian A, Zali M R. Evaluation of the Aassociation Between Programmed Cell Death 1 Gene Single Nucleotide Polymorphism +7146A/G and Susceptibility to Chronic Hepatitis B . AMUJ. 2015; 18 (7) :66-74
URL: http://amuj.arakmu.ac.ir/article-1-3589-en.html


Volume 18, Issue 7 (10-2015) Back to browse issues page
مجله دانشگاه علوم پزشکی اراک Arak Medical University Journal
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