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:: Volume 18, Number 7 (10-2015) ::
amuj 2015, 18(7): 66-74 Back to browse issues page
Evaluation of the Aassociation Between Programmed Cell Death 1 Gene Single Nucleotide Polymorphism +7146A/G and Susceptibility to Chronic Hepatitis B
Seyed Reza Mohebbi , Hamed Naghoosi, Pedram Azimzadeh, Shaghayegh Derakhshani, Afsaneh Sharifian, Mohammad Reza Zali
Assistant professor Basic Sciences and Epidemiology of Gastroenterology Diseases Research Center, Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Siences, Tehran, Iran. , srmohebbi@gmail.com
Abstract:   (2447 Views)

  Background: In spite of designing and applying an effective vaccine against Hepatitis B virus (HBV), chronic infection with this virus is still one of the most important health problems worldwide. Host genetic background including single nucleotide polymorphisms play a significant role in chronicity or clearance of the infection. The final product of programmed cell death 1 gene (PDCD1) is expressed frequently on T-cells and in chronic viral infections, prevent the virus-specific T-cell response against the virus. In this study, the association of a single nucleotide polymorphism (+7146A/G) in intron 4 of PD1 gene with chronic hepatitis B infection in Iranian population has been assessed.

  Materials and Methods: 212 chronic HBV patients and 208 healthy controls were analyzed in this case-control study. Genomic DNA of the studied individuals was extracted and after performing polymerase chain reaction (PCR), polymorphism of +7146 was determined via RFLP method.

  Results: Frequencies of GG, GA and AA genotypes on position 7146 of the intron 4 of PD1 gene were 77.4%, 20.7% and 1.9% in patient group and 80.8%, 15.4% and 3.8% in control group, respectively. After statistical analysis, No significant difference was observed between patient and control groups (p=0.198).

  Conclusion: Genotype frequencies in the studied population are in accordance with the results of previous studies. Results of the present study suggest that there is not any association between A/G single nucleotide polymorphism in intron 4 of PD1 gene and susceptibility to chronic hepatitis B in Iranian population.

Keywords: Chronic infection, Hepatitis B, Human PDCD1 protein, Polymorphism, Single Nucleotide
Full-Text [PDF 654 kb]   (460 Downloads)    
Type of Study: Original Atricle | Subject: Infection
Received: 2015/03/26 | Accepted: 2015/05/20 | Published: 2015/09/22
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Mohebbi S R, Naghoosi H, Azimzadeh P, Derakhshani S, Sharifian A, Zali M R. Evaluation of the Aassociation Between Programmed Cell Death 1 Gene Single Nucleotide Polymorphism +7146A/G and Susceptibility to Chronic Hepatitis B . amuj. 2015; 18 (7) :66-74
URL: http://amuj.arakmu.ac.ir/article-1-3589-en.html
Volume 18, Number 7 (10-2015) Back to browse issues page
مجله دانشگاه علوم پزشکی اراک Arak Medical University Journal
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