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:: Volume 18, Number 5 (8-2015) ::
amuj 2015, 18(5): 21-32 Back to browse issues page
Passive Immunity with Recombinant Anti-Pseudomonas Aeruginosa Type B Fagellin Antibody in a Burned Mouse Model
Bahador Behrouz, Nour Amirmozafari , Mohammad Mehdi Fizabadi, Nima Khoramabadi, Mahboobeh Bahroudi, Mehdi Mahdavi
Associate Professor, Department of Microbiology, Iran University of Medical Sciences, Tehran, Iran , amirmozafari@yahoo.com
Abstract:   (4345 Views)

Background: Pathogenic Pseudomonas aeruginosa strains produce a polar flagellum that required for motility, adhesion, invasion and secretion of virulence factors. The aim of this study was to evaluate the effect of prevention and treatment with anti-recombinant type B flagellin antibody in a burned mouse model.

Materials and Methods: One hundred twenty six mice were divided into 16 groups injected with different regimen of anti-recombinant type B flagellin antibody. Infections were caused by sub-dermal injection of P. aeruginosa PAO1 and PAK strains at the burn site. Groups were monitored for mortality for one week. Additionally, functional activity of this antibody was assessed by motility inhibition and opsonophagocytosis assays.

Results:  Immunotherapy with rabbit antisera substantially increased (85.71%) survival rate of mice challenged with a homologous strain PAO1 compared with the control PBS. The mortality rate in mice infected by the heterologous strain PAK was only 28.57%. This antibody increased phagocytosis killing of the homologous strain but it only had a slight effect on the heterologous strain.

Conclusion:  Passive immunotherapy protected burned mice challenged with the homologous strain but showed lower efficacy against the heterologous strain.

Keywords: Burn, Passive immunotherapy, Pseudomonas aeruginosa, Recombinant flagellin
Full-Text [PDF 1015 kb]   (3352 Downloads)    
Type of Study: Original Atricle | Subject: Infection
Received: 2015/01/18 | Accepted: 2015/04/15 | Published: 2015/07/22
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Behrouz B, Amirmozafari N, Fizabadi M M, Khoramabadi N, Bahroudi M, Mahdavi M. Passive Immunity with Recombinant Anti-Pseudomonas Aeruginosa Type B Fagellin Antibody in a Burned Mouse Model. amuj. 2015; 18 (5) :21-32
URL: http://amuj.arakmu.ac.ir/article-1-3436-en.html
Volume 18, Number 5 (8-2015) Back to browse issues page
مجله دانشگاه علوم پزشکی اراک Arak Medical University Journal
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