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:: Volume 17, Issue 12 (3-2015) ::
amuj 2015, 17(12): 1-8 Back to browse issues page
The Effective of Cord Blood Platelet Extraction (PL) on Meiosis Resumption and Maturation of Mouse Oocyte at Germinal Vesicle stage (GV)
Hasan Pazoki , Hosein Imani , Abdolhosein Shahverdi , Farah Farokhi , Leila Sadat Tahaei
MSc, Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran , hasan_fsh33@yahoo.com
Abstract:   (4359 Views)

Background: Maturation of oocytes and fertilization in vitro can be considered as one of the most important steps to treat infertility. In this study maturation medium was enriched with Platelet extraction (PL) which has high concentration of growth factors. Meiosis resumption and maturation was monitored after 18 hours of maturation.

Materials and Methods: In this experimental study, oocytes at germinal vesicle stage were obtained from mature NMRI female mice. Maturation medium was &alphaMEM and the control groups had 5-10% FBS and the medium in the experimental groups was enriched by 5, 10% PL and the combination of 5% PL and 5% FBS. Meiosis resumption and maturation were observed after 18 hours.

Results: The rate of matured oocytes in the experimental group 5% PL for both COC and DO group was significantly higher than the control groups (P<0.05). The maturation rate for 5% PL was 61% for the COC group and 72% for the DO group while this rate for 5% FBS control group was 53% and 50% respectively.

Conclusion: PL had a significant effect on meiosis resumption and maturation of oocyte at germinal vesicle stage. Based on these results, PL could be used as a maturation promoting factor.

Keywords: Cumulus oocyte complex (COC), Denuded oocyte (DO), Fetal bovine serum (FBS), Germinal vesicle (GV), In vitro maturation (IVM), Platelet layset (PL)
Full-Text [PDF 590 kb]   (753 Downloads)    
Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2014/10/5 | Accepted: 2014/11/11 | Published: 2015/02/18
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Pazoki H, Imani H, Shahverdi A, Farokhi F, Tahaei L S. The Effective of Cord Blood Platelet Extraction (PL) on Meiosis Resumption and Maturation of Mouse Oocyte at Germinal Vesicle stage (GV). amuj. 2015; 17 (12) :1-8
URL: http://amuj.arakmu.ac.ir/article-1-3206-en.html
Volume 17, Issue 12 (3-2015) Back to browse issues page
مجله دانشگاه علوم پزشکی اراک Arak Medical University Journal
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