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:: Volume 17, Issue 8 (11-2014) ::
AMUJ 2014, 17(8): 27-34 Back to browse issues page
To Assay the Relation between the Vascular Endothelial Growth Factor Expression and Breast Cancer Tumor Formation
Morteza Sadeghi , Zohreh Hojati , Kamran Ghaedi
Associate Professor, Depatment of biology, university of Istahan, Isfahan , Iran , zh.hojati@sci.ui.ac.ir
Abstract:   (3444 Views)

Background: Vascular endothelial growth factor (vegf) is one of the most important regulator of angiogenesis, there are some reports about the relation of VEGF over expression and progression of tumor in several cancers. The aim of this study is assay of four VEGF isoforms expression in breast cancer tumor samples.

Materials and Methods: 25 breast cancer tumor samples and 25 health samples were used in this study, mRNA was extracted from each sample and then cDNA was made. The expression of four isoforms VEGF121, VEGF165, VEGF183 and VEGF189 was measured by real time reverse transcription PCR (RT-PCR) and gel electrophoresis.

Results: Among the four isoforms, VEGF165 and VEGF 121 had maximum and VEGF 183 and VEGF 189 had minimum expression level in all samples. The total expression level of VEGF had a significant increase in tumor samples in comparison with the control samples (4/6, p<0.01).

Conclusion: There is a significant relation between the VEGF over expression and breast cancer tumor formation, which it can be used as a prognosis marker of breast cancer in future.

Keywords: Breast cancer, Gene expression pattern analysis, Vascular endothelial growth factor
Full-Text [PDF 947 kb]   (1082 Downloads)    
Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2014/08/20 | Accepted: 2014/08/30 | Published: 2014/10/22
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Sadeghi M, Hojati Z, Ghaedi K. To Assay the Relation between the Vascular Endothelial Growth Factor Expression and Breast Cancer Tumor Formation. AMUJ. 2014; 17 (8) :27-34
URL: http://amuj.arakmu.ac.ir/article-1-3126-en.html

Volume 17, Issue 8 (11-2014) Back to browse issues page
مجله دانشگاه علوم پزشکی اراک Arak Medical University Journal
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