[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 17, Number 8 (11-2014) ::
amuj 2014, 17(8): 27-34 Back to browse issues page
To Assay the Relation between the Vascular Endothelial Growth Factor Expression and Breast Cancer Tumor Formation
Morteza Sadeghi, Zohreh Hojati , Kamran Ghaedi
Associate Professor, Depatment of biology, university of Istahan, Isfahan , Iran , zh.hojati@sci.ui.ac.ir
Abstract:   (3185 Views)

Background: Vascular endothelial growth factor (vegf) is one of the most important regulator of angiogenesis, there are some reports about the relation of VEGF over expression and progression of tumor in several cancers. The aim of this study is assay of four VEGF isoforms expression in breast cancer tumor samples.

Materials and Methods: 25 breast cancer tumor samples and 25 health samples were used in this study, mRNA was extracted from each sample and then cDNA was made. The expression of four isoforms VEGF121, VEGF165, VEGF183 and VEGF189 was measured by real time reverse transcription PCR (RT-PCR) and gel electrophoresis.

Results: Among the four isoforms, VEGF165 and VEGF 121 had maximum and VEGF 183 and VEGF 189 had minimum expression level in all samples. The total expression level of VEGF had a significant increase in tumor samples in comparison with the control samples (4/6, p<0.01).

Conclusion: There is a significant relation between the VEGF over expression and breast cancer tumor formation, which it can be used as a prognosis marker of breast cancer in future.

Keywords: Breast cancer, Gene expression pattern analysis, Vascular endothelial growth factor
Full-Text [PDF 947 kb]   (902 Downloads)    
Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2014/08/20 | Accepted: 2014/08/30 | Published: 2014/10/22
Send email to the article author

Add your comments about this article
Your username or email:

Write the security code in the box >



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Sadeghi M, Hojati Z, Ghaedi K. To Assay the Relation between the Vascular Endothelial Growth Factor Expression and Breast Cancer Tumor Formation. amuj. 2014; 17 (8) :27-34
URL: http://amuj.arakmu.ac.ir/article-1-3126-en.html
Volume 17, Number 8 (11-2014) Back to browse issues page
مجله دانشگاه علوم پزشکی اراک Arak Medical University Journal
Persian site map - English site map - Created in 0.056 seconds with 790 queries by yektaweb 3506