Volume 17, Issue 8 (11-2014)                   J Arak Uni Med Sci 2014, 17(8): 27-34 | Back to browse issues page

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Sadeghi M, Hojati Z, Ghaedi K. To Assay the Relation between the Vascular Endothelial Growth Factor Expression and Breast Cancer Tumor Formation. J Arak Uni Med Sci. 2014; 17 (8) :27-34
URL: http://amuj.arakmu.ac.ir/article-1-3126-en.html
Associate Professor, Depatment of biology, university of Istahan, Isfahan , Iran , zh.hojati@sci.ui.ac.ir
Abstract:   (3870 Views)

Background: Vascular endothelial growth factor (vegf) is one of the most important regulator of angiogenesis, there are some reports about the relation of VEGF over expression and progression of tumor in several cancers. The aim of this study is assay of four VEGF isoforms expression in breast cancer tumor samples.

Materials and Methods: 25 breast cancer tumor samples and 25 health samples were used in this study, mRNA was extracted from each sample and then cDNA was made. The expression of four isoforms VEGF121, VEGF165, VEGF183 and VEGF189 was measured by real time reverse transcription PCR (RT-PCR) and gel electrophoresis.

Results: Among the four isoforms, VEGF165 and VEGF 121 had maximum and VEGF 183 and VEGF 189 had minimum expression level in all samples. The total expression level of VEGF had a significant increase in tumor samples in comparison with the control samples (4/6, p<0.01).

Conclusion: There is a significant relation between the VEGF over expression and breast cancer tumor formation, which it can be used as a prognosis marker of breast cancer in future.

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Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2014/08/20 | Accepted: 2014/08/30 | Published: 2014/10/22

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