[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 17, Number 9 (12-2014) ::
amuj 2014, 17(9): 77-85 Back to browse issues page
Time Dependent Hippocampal Glutamate and GABA Change Following Pentylenetetrazol Induced Convulsions in Rat
Elham Goudarzi, Mahmoud Elahdadi Salmani , Taghi Lashkar Boluki, Iran Goudarzi
Asist Professor, Faculty of Biology, Damghan University, Damghan, Iran , elahdadi@du.ac.ir
Abstract:   (2326 Views)

Background: Seizure is an abnormal electrical activity probably due to an imbalance between excitation and inhibition in the brain. Pentylenetetrazol (PTZ) is a chemical convulsive agent abundantly used in laboratory animals. PTZ induces a change in glutamate and GABA in the brain which this study investigates the persistence of this change.

Materials and Methods: In this experimental study, 18 male Wistar rats divided into 3 groups. Three i.v doses of PTZ 20, 25 and 30 mg/ml were used to determine the effective PTZ dose. Convulsive behaviors were monitored as tonic clonic and myoclonic twitches. Hippocampal glutamate and GABA contents were measured using a biochemical method.

Results: Dose of 20 was resulted in long latency to and short lasting TC convulsions with a high volume of injected PTZ solution. On the other hand, dose of 25 and 30 led to short latency and long lasting convulsions with low volume of injecting solution. However there was high rate of mortality (100%) in dose of 30 mg/ml. Hippocampal glutamate content was decreased in zero and 20 min groups while GABA content was decreased only in 20 min group.

Conclusion: It is concluded that dose of 25 is the appropriate i.v dose to induce TC convulsions in rats which decreases glutamate and GABA while increases the ratio of glutamate to GABA. Therefore, alteration of glutamate and GABA may be the basis for subsequent seizure induced changes.

Keywords: GABA, Glutamate, Pentylenetetrazol, Seizure, Time
Full-Text [PDF 650 kb]   (525 Downloads)    
Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2014/07/13 | Accepted: 2014/09/15 | Published: 2014/11/19
Send email to the article author

Add your comments about this article
Your username or email:

Write the security code in the box >



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Goudarzi E, Elahdadi Salmani M, Lashkar Boluki T, Goudarzi I. Time Dependent Hippocampal Glutamate and GABA Change Following Pentylenetetrazol Induced Convulsions in Rat. amuj. 2014; 17 (9) :77-85
URL: http://amuj.arakmu.ac.ir/article-1-3067-en.html
Volume 17, Number 9 (12-2014) Back to browse issues page
مجله دانشگاه علوم پزشکی اراک Arak Medical University Journal
Persian site map - English site map - Created in 0.057 seconds with 791 queries by yektaweb 3503