Volume 17, Issue 4 (7-2014)                   J Arak Uni Med Sci 2014, 17(4): 11-19 | Back to browse issues page

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Bahadori M, Zafar Balanezjad S, Forghanifard M M. Expressional Analysis of SALL4 mRNA in Prosencephalon during Chicken Embryogenesis. J Arak Uni Med Sci. 2014; 17 (4) :11-19
URL: http://amuj.arakmu.ac.ir/article-1-2772-en.html
Assistant Professor, Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran , Forghanifard@gmail.com
Abstract:   (7839 Views)

Background: According to the important of SALL4 gene during the development of embryonic neurvous system, our aim in this study was to analyze and quantify mRNA expression of SALL4 in Prosencephalon during different stages of chicken embryogenesis.

Materials and Methods: In this experimental study, incubated Ross fertilized eggs were applied in 37°C-37.5°C in 60-65% humidified atmosphere after beginning of embryogenesis. Prosencephalon part of the brain tissue was collected from the eggs, daily. Total RNA extraction and cDNA synthesis was performed from resected tissues. The synthesized cDNA was used as template for quantitatively analysis of SALL4 mRNA expression by real-time PCR.

Results: The Results indicate that the level of SALL4 gene expression is significantly variable during embryogenesis. However it doesn’t show variation during the early days. The maximum copy number of SALL4 mRNA was quantified on 15 th day of chicken development.

Conclusion: SALL4 mRNA expression is high when the Prosencephalon is under development, using of HAMBURGER–HAMILTON chart, there is relation between increasing SALL4 expression and developing limbs and anterior brain.

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Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2014/01/24 | Accepted: 2014/05/7 | Published: 2014/06/21

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