Volume 16, Issue 10 (1-2014)                   J Arak Uni Med Sci 2014, 16(10): 11-18 | Back to browse issues page

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Baazm M, Darabi M R, Babaie S, Talebi R. Improvement in Sperm Parameters with Gonadotropin-Releasing Hormone Analogue in Busulfan Treated Prepubertal Mice . J Arak Uni Med Sci. 2014; 16 (10) :11-18
URL: http://amuj.arakmu.ac.ir/article-1-2474-en.html
Assistant Professor, Department of anatomy, Arak University of Medical Sciences, Arak, Iran , dr.baazm@arakmu.ac.ir
Abstract:   (6976 Views)

Background: One of the side effects of chemotherapy agents is gonadal suppression leading to infertility. The present study examined the feasibility of using a gonadotropin releasing hormone agonist (GnRH-A) in the prevention of busulfan induced azoospermia in prepubertal mice.

Materials and Methods: In this case and control research, Leuprolide (3.8 mg/kg) was given to prepubertal male mice 2 weeks after or prior to busulfan (40 mg/kg) administration. The animals received one or two doses of leuprolide after busulfan administration with 4 weeks apart. Six weeks after the last leuprolide treatment, the sperm parameters were analyzed icluded sperm count and motility according to the World Health Organization criteria.

Results: At the time of scarifies, testicular weight were increased in animals receiving leuprolide. In animals receiving leuprolide pretreatment, the percentage of progressive and immotile sperm was significantly (p&le0.05) increased and decreased respectively. But the percentage of non progressive sperm did not change. The sperm motility in busulfan treated animals with post treatment didn't change. The sperm count also increased in pretreatment animals but it was not significant (p&ge0.05).

Conclusion: The present study demonstrates that leuprolide pretreatment resulted in improvement in sperm motility in prepubertal male mice.

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Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2013/08/18 | Accepted: 2013/11/20 | Published: 2013/12/18

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