[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 11, Number 2 (6-2008) ::
amuj 2008, 11(2): 94-101 Back to browse issues page
Comparison of agar screen and duplex-PCR in determination of methicillin resistant Staphylococcus aureus (MRSA) strains isolated from nose of personnel in Hajar hospital of Shahre-kord, 2007
Mohammad reza Nafisi , Huriyeh Kalhor, Behzad Zamanzad, Ali Karimi, Efat Farokhi, Majid Validi
Assistant professore , mrnafisi@yahoo.com
Abstract:   (17554 Views)
Introduction: Methicillin resistant staphylococcus aureus strains are the most important agents of nosocomial infections. The conventional antibiotic susceptibility methods such as disk diffusion are not suitable for detection of these strains due to their heteroresistancy. Therefore, in this study, agar screen and duplex-PCR were compared in determination of methicillin resistant Staphylococcus aureus (MRSA) strains isolated from nose of personnel in Hajar hospital of Shahre-kord, 2007. Materials and Methods: In this experimental study a total of 204 nasal swabs from personnel of Hajar hospital over a period of 6 months were collected. The specimens were cultured on mannitol salt agar for primary isolation and identification of Staphylococcus aureus strains and their susceptibility pattern to oxacillin was assessed using agar screen method. Finally, using duplex PCR, the isolates were tested for the presence of mecA gene. Results were compared and sensitivity and specificity of the method was determined. Results: In this study, 23 of the 52 (44%) Staphylococcus aureus isolates were resistant to oxacillin using agar screen method. However, mecA gene was detected in 27 of the 52 strains (52%). Our results showed that the sensitivity and specificity of agar screen method in determination of MRSA strains were 81.5% and 96%, respectively comparing with PCR. Conclusion: Oxacillin agar screen, comparing PCR, is an inexpensive, applied and phenotypical method with low false positive and suitable for screening of MRSA. However, due to its relatively high false negative results is not appropriate for screening of MRSA strains isolated from hospital-employed nasal carriers.
Keywords: Nosocomial infection, methicillin resistance, gene, polymerase chain
Full-Text [PDF 267 kb]   (2855 Downloads)    
Subject: General
Received: 2009/03/10
Send email to the article author

Add your comments about this article
Your username or email:

Write the security code in the box >



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Nafisi M R, Kalhor H, Zamanzad B, Karimi A, Farokhi E, Validi M. Comparison of agar screen and duplex-PCR in determination of methicillin resistant Staphylococcus aureus (MRSA) strains isolated from nose of personnel in Hajar hospital of Shahre-kord, 2007. amuj. 2008; 11 (2) :94-101
URL: http://amuj.arakmu.ac.ir/article-1-230-en.html
Volume 11, Number 2 (6-2008) Back to browse issues page
مجله دانشگاه علوم پزشکی اراک Arak Medical University Journal
Persian site map - English site map - Created in 0.056 seconds with 799 queries by yektaweb 3506