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:: Volume 16, Issue 1 (4-2013) ::
AMUJ 2013, 16(1): 79-86 Back to browse issues page
The Neuroprotective Effects of Hydroalcoholic Extract of Nigella Sativa on Alpha Motoneurons Degeneration After Sciatic Nerve Injury in Rats
Mahboobe Ferdosi Makan , Jina Khayatzade , Maryam Tehranipoor , Morteza Behnam Rasooli
Assistant Professor, Department of Biology, Islamic Azad University, Mashhad Branch, Mashhad, Iran , maryam_tehranipour@mshdiau.ac.ir
Abstract:   (7269 Views)

Background: Peripheral nervous system damages reverse as retrograde to alpha neuron cell bodies and cause spinal degeneration. The fact that herbs, due to their antioxidant properties, have an important role in viability and reproduction of neurons has led to the application of their extracts. Hence, this study was done to determine the neuro-protective effects of the hydroalcoholic extract of Nigella sativa on alpha-motoneurons degeneration after sciatic nerve injury in rats.

Materials and Methods: In this experimental study, 24 male Wistar rats with average bodyweight of 250-300gr were divided into four groups of six: Control, compression, A (compression+hydroalcoholic extract 50 mg/kg), and B (compression+hydroalcoholic extract 75 mg/kg). In compression and treatment groups, the right leg sciatic nerve was subjected to compression (30 seconds). In treatment groups, the extract was injected intraperitoneally two times after compression. After 28 days, lumbar segments of spinal cord, L2-L4, were sampled through perfusion method. After going through tissue passage stages, they were cut in serial sections (7µ) and stained with toluidine blue. Then the density of alpha motoneurons of the spinal cord ventral horn was measured by dissector method.

Results: Neuronal density showed a significant difference between the compression and control groups (p<0.05). Also, in treatment groups A and B, it had a significant increase compared to the compression group (p<0.05).

Conclusion: The results indicated that the hydroalcoholic extract of Nigella sativa has neuro-protective effects and the increase in neuronal density is relevant to the amount of extract used.

Keywords: Nerve degeneration, Nigella sativa, Rat, Sciatic nerve
Full-Text [PDF 379 kb]   (1332 Downloads)    
Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2012/08/6 | Accepted: 2013/08/28 | Published: 2013/08/28
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Ferdosi Makan M, Khayatzade J, tehranipoor M, Behnam Rasooli M. The Neuroprotective Effects of Hydroalcoholic Extract of Nigella Sativa on Alpha Motoneurons Degeneration After Sciatic Nerve Injury in Rats. AMUJ. 2013; 16 (1) :79-86
URL: http://amuj.arakmu.ac.ir/article-1-1883-en.html

Volume 16, Issue 1 (4-2013) Back to browse issues page
مجله دانشگاه علوم پزشکی اراک Arak Medical University Journal
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