[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 15, Number 10 (March 2013) ::
amuj 2013, 15(10): 85-94 Back to browse issues page
The effect of atrazine on spermic parameters and fertility potential in mature rats
Gholamreza Najafi , Rahim Hobe Naghi, Aref Hoshyari, Masoud Moghadaszadeh, Behzad Ghorbanzadeh
Asistant Profesor Urmia University , g.najafi2006@yahoo.com
Abstract:   (7335 Views)
Background: Atrazine is an herbicide used widely by farmers in controlling weeds. The aim of this investigation was to evaluate the effect of atrazine, as an herbicide, on sperm quality, sperm DNA damage, invitrofertilization (IVF), and embryonic development in mature male rats. Materials and Methods: In this experimental study, 42 mature male Wistar rats weighing 170±5g were divided into three groups, including one control and two treatment groups. The rats in the control group were administered corn oil (0.2 ml/day) and the rats in the test groups were orally gavaged with atrazine 150mg/kg (high dose) and 75mg/kg (low dose) body weight daily for a total of 45 days. Epidydimis tail was cut and placed in 1 ml of human tubular fluid (HTF) medium for 30 minutes in an atmosphere of 5% CO2 in air at 370C. The sperms were analyzed for sperm count, sperm viability, motility, DNA damage, immature sperm, and in vitro fertilization. Data were analyzed by One-Way ANOVA. Results: In this study, atrazine provoked a significant decrease (P<0.05) in sperm number, sperm viability, and sperm motility. The data suggest that the atrazine had a negative impact on sperm maturation and DNA integrity in a time-dependent manner, which consequently caused a significantly remarkable reduction in IVF ability (P<0.05). Conclusion: Atrazine is capable of inducing DNA damage and chromatin abnormalities of spermatozoa which can contribute to a low fertilization rate.
Keywords: Atrazine, rats, spermatozoa
Full-Text [PDF 432 kb]   (1451 Downloads)    
Subject: Basic Sciences
Received: 2012/02/18 | Accepted: 2012/09/18
Send email to the article author

Add your comments about this article
Your username or email:

Write the security code in the box >



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Najafi G, Hobe Naghi R, Hoshyari A, Moghadaszadeh M, Ghorbanzadeh B. The effect of atrazine on spermic parameters and fertility potential in mature rats. amuj. 2013; 15 (10) :85-94
URL: http://amuj.arakmu.ac.ir/article-1-1557-en.html
Volume 15, Number 10 (March 2013) Back to browse issues page
مجله دانشگاه علوم پزشکی اراک Arak Medical University Journal
Persian site map - English site map - Created in 0.056 seconds with 796 queries by yektaweb 3506