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:: Volume 14, Number 7 (Brucellosis Supplement 2012) ::
amuj 2012, 14(7): 40-48 Back to browse issues page
Evaluation of three DNA extraction methods for detection of brucella DNA in human serum samples
Haadi Peeridogaheh, Zahra valinezhad, Farhad Pourfarzi
Associate professor Department of Community Medicine, School of Medicine, Ardabil University of Medical Sciences , f.pourfarzi@arums.ac.hr
Abstract:   (10442 Views)
Background: Human brucellosis is a significant public health concern in many countries, including Iran. Therefore, the development of new diagnostic techniques, with high sensitivity and minimum risk of laboratory infection are of great importance. PCR is one of the procedures which has these advantages. However, PCR efficiency is largely dependent on DNA extraction methods. In this study, we studied the efficiency of three different extraction methods of brucella DNA in serum samples. Materials and Methods: In this experimental study, microbial suspensions were initially prepared in saline that its turbidity was equivalent to 0.5 McFarland. Human serum samples were spiked with certain concentrations of Brucella melitensis in vitro. DNA was extracted by three methods and tested by a genus-specific PCR method. Results: Our results showed that the cinneagen kit protocol detected brucella DNA in lower serum concentrations compared with the other protocols. Cinnagen kit could detect brucella DNA in ten-fold dilution in comparison with the other two methods. Conclusion: According to the findings of this study, cinnagen kit was the preferred assay method that yields a better sensitivity for isolation of brucella DNA in serum samples.
Keywords: Brucellosis, DNA extraction, PCR
Full-Text [PDF 1787 kb]   (1689 Downloads)    
Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2011/11/1 | Accepted: 2012/01/7
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Peeridogaheh H, valinezhad Z, Pourfarzi F. Evaluation of three DNA extraction methods for detection of brucella DNA in human serum samples. amuj. 2012; 14 (7) :40-48
URL: http://amuj.arakmu.ac.ir/article-1-1384-en.html
Volume 14, Number 7 (Brucellosis Supplement 2012) Back to browse issues page
مجله دانشگاه علوم پزشکی اراک Arak Medical University Journal
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