Volume 14, Issue 7 (Brucellosis Supplement 2012)                   J Arak Uni Med Sci 2012, 14(7): 1-8 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Abtahi H, Salmanian A H, Rafati S, Mossayebi G, Amouzande A R. Evaluation of the immunogenicity of Brucella abortus P39 gene in Balb/c mice. J Arak Uni Med Sci. 2012; 14 (7) :1-8
URL: http://amuj.arakmu.ac.ir/article-1-1352-en.html
Assistant Professor Molecular and Medical Research Center, Arak University of Medical Sciences, Arak, Iran , h_abtahi2@yahoo.co.uk
Abstract:   (10569 Views)
Background: Brucella is a gram-negative intracellular bacterium. Since Brucella brings about health and socio-economic problems, its control is of primary importance. The common method of vaccination includes using live attenuated strains of this bacterium. This study was done to evaluate the immunogenicity of Brucella aburtus P39 gene in Balb/c mice. Materials and Methods: In this experimental study, P39 gene was amplified by polymerase chain reaction (PCR) method and after extraction, it was sub-cloned to eukaryotic expression vector pcDNA3. The intramuscular injection of the obtained plasmid to the Balb/c mice was done in three stages. After the last vaccination, immunologic tests, such as proliferative response in lymphocytes, IFN- assessment, IL-5, and determination of IgG2a and IgG1, were run. Results: The level of activation in splenic lymphocytes response was 3.6 and the measured IFN- was 3 ng/ml, whereas IL-5 was insignificant. IgG2a and IgG1 titers were 1.640 and 1.40, respectively. Conclusion: The findings of the immunological analysis show the appropriate immune response in Balb/c mice model after the injection of P39 gene containing plasmid. The immune system response was in Th1 form which decreased the number of bacteria in spleen. Therefore, P39 gene is of appropriate immunogenicity and DNA vaccination is efficient in the activation of cell immune response against this bacterium.
Full-Text [PDF 1853 kb]   (1735 Downloads)    
Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2011/10/9 | Accepted: 2011/12/29

Add your comments about this article : Your username or Email:
Write the security code in the box

Send email to the article author

© 2018 All Rights Reserved | Journal of Arak University of Medical Sciences

Designed & Developed by : Yektaweb