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:: Volume 14, Number 7 (Brucellosis Supplement 2012) ::
amuj 2012, 14(7): 55-63 Back to browse issues page
Evaluation of the antibacterial effects of Eucalyptus globulus extracts on intramacrophage Brucella melitensis 16M
Peyman Abdollahzadeh, Reza Shapouri , Shahrzad Nasiri Semnani, Hamed Alizadeh
Assistant Prof Islamic azad university-zanjan branch , rezashapoury@yahoo.com
Abstract:   (14687 Views)
Background: Brucellosis is caused by brucella which is a facultative intracellular pathogen invading both professional and nonprofessional phagosytic cells. Eucalyptus globulus is one of the most widely used medicinal plants in folk medicine throughout the world. The aim of this study was to evaluate the antibacterial effects of Eucalyptus globulus extracts on intramacrophage Brucella melitensis 16M. Materials and Methods: In this experimental study, after preparing aquatic, ethanolic, and acetonic extracts of Eucalyptus globules, the effect of the extracts on intramacrophge survival of B. melitensis 16M obtained from cell culture of Balb/c mice peritoneal macrophages was studied. In order to do this, after lysis of macrophages, through preparation of serial dilutions and culture on Mueller Hinton agar medium, the number of colonies grown was counted. Results: The maximum antimicrobial activity of Eucalyptus globulus extracts on intramacrophage B. melitensis 16 M were in 1:40 dilution (21.62 mg/ml) of the aquatic extract, 1: 640 dilution (1.26 mg/ml) of the ethanolic extract, and 1:320 dilution (2.59 mg/ml) of the acetonic extract after 24h. Conclusion: Aquatic, acetonic, and ethanolic extracts of Eucalyptus globulus possess antimicrobial properties against intramacrophage B. melitensis 16M and ethanolic extract has the most effective antimicrobial activity on intramacrophage Brucella melitensis therefore, these extracts can be useful in treatment of brucellosis.
Keywords: Antibacterial activity, Brucella melitensis 16M, Cell culture, Eucalyptus globulus extracts, Macrophage
Full-Text [PDF 1956 kb]   (2063 Downloads)    
Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2011/10/6 | Accepted: 2012/01/31
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Abdollahzadeh P, Shapouri R, Nasiri Semnani S, Alizadeh H. Evaluation of the antibacterial effects of Eucalyptus globulus extracts on intramacrophage Brucella melitensis 16M. amuj. 2012; 14 (7) :55-63
URL: http://amuj.arakmu.ac.ir/article-1-1348-en.html
Volume 14, Number 7 (Brucellosis Supplement 2012) Back to browse issues page
مجله دانشگاه علوم پزشکی اراک Arak Medical University Journal
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