[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 20, Issue 12 (3-2018) ::
AMUJ 2018, 20(12): 35-43 Back to browse issues page
Examining the Effect of Inhibition of Lysyl oxidase on Involved Genes in Hypertrophy Following One Period of Resistance Training in Wistar Rats
Moein Rahimi Sadegh , Amir Rashidlamir , Ali Akbar Haddad Mashhad Rizeh , Mohammad Soukhtanloo
Associate Professor, Department of Exercise Physiology, Faculty of Sport Sciences, Ferdowsi University of Mashhad, Mashhad, Iran , rashidamir@um.ac.ir
Abstract:   (38 Views)
Abstract
Background: Recent Research has pointed to the involvement of lysyl oxidase (LOX) in the muscle development. Despite the fact, there is currently no direct evidence that lox is involved in the myogenic factors and exercise-induced hypertrophy. Therefore, the purpose of this study was to investigate the effect of lysyl oxidase inhibition on exercise-induced hypertrophy, as well as the gene expression of MyoD1, myogenin, TGF-β and LOX in FHL muscle of wistar rats.
Material and Methods: In this experimental research, 32 male Wister rats with an average weight of 220±15 were divided into four groups: resistance training, normal control, treated control and treated resistance training. Rats performed a resistance training for 8 weeks in which animals climbed a vertical ladder of 1­m and inclined at 85° with weights attached to their tails. In order to inhibit lysyl oxidase, treatment group rats received daily injection of intraperitoneal β-aminopropionitrile (120 mg/kg/day). FHL muscle was extracted to measure relative muscle weight, as well as genes expression of MyoD1, myogenin, TGF-β and LOX by real time-PCR.
Results: Relative weight of FHL muscle was decreased significantly in the treated groups with BAPN, compared with normal groups (p<0.05). Lysyl oxidase showed a significant increase compared to the normal control group following a resistance training (p<0.05). LOX gene expression in treatment groups showed a significant increase compared to normal control group (p<0.05). TGF-β gene expression in BAPN-treated groups significantly was increased (p<0.05) and Myogenin in treatment groups showed a significant decrease compared to normal control groups (p<0.05). The expression of MyoD1 gene in treatment groups was only decreased significantly in the treated control groups compared to normal control groups (p<0.05).
Conclusion: Results showed that inhibiting the lysine oxidase enzyme could affect the genes involved in hypertrophy and reduce the hypertrophy induced by resistance training.

 
Keywords: Hypertrophy, Lysyl oxidase, Resistance exercise, Skeletal muscle, β-aminopropionitrile
Full-Text [PDF 841 kb]   (21 Downloads)    
Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2017/09/16 | Accepted: 2018/01/14 | Published: 2018/02/14
Send email to the article author

Add your comments about this article
Your username or Email:

Write the security code in the box >


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Rahimi Sadegh M, Rashidlamir A, Haddad Mashhad Rizeh A A, Soukhtanloo M. Examining the Effect of Inhibition of Lysyl oxidase on Involved Genes in Hypertrophy Following One Period of Resistance Training in Wistar Rats. AMUJ. 2018; 20 (12) :35-43
URL: http://amuj.arakmu.ac.ir/article-1-5319-en.html


Volume 20, Issue 12 (3-2018) Back to browse issues page
مجله دانشگاه علوم پزشکی اراک Arak Medical University Journal
Persian site map - English site map - Created in 0.062 seconds with 802 queries by yektaweb 3600